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cultrex ultimatrix reduced growth factor basement membrane extract  (R&D Systems)


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    R&D Systems cultrex ultimatrix reduced growth factor basement membrane extract
    Cultrex Ultimatrix Reduced Growth Factor Basement Membrane Extract, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cultrex+ultimatrix+reduced+growth+factor+basement+membrane+extract/Cultrex+RGF+Basement+Membrane+Extract/pm42017661-114-7-15
    Average 94 stars, based on 35 article reviews
    cultrex ultimatrix reduced growth factor basement membrane extract - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Membrane:

    Article Title: Antibody-based delivery of interleukin-2 modulates the immunosuppressive tumor microenvironment and achieves cure in pancreatic ductal adenocarcinoma syngeneic mice
    Article Snippet: .. Briefly, 2D cell lines were detached using trypsinization and suspended in 50 μl of Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (R&D systems Cat# BME001-10) at a density of 100,000 cells per dome. .. The spheroids were then grown in PancreaCultTM Organoid Growth Mouse Medium (STEMCELL Technologies, Cat#06040) and inspected daily.

    Article Title: Colorectal cancer patients-derived immunity-organoid platform unveils cancer-specific tissue markers associated with immunotherapy resistance.
    Article Snippet: Digestion was blocked with Washing Medium (DMEM F12 Advanced - 12634-010, Gibco, 15 mM Hepes - 15630-080, Gibco, 1% BSA - A1391, Applichem) then filtered through a 70 μm strainer. .. Tumoral crypts were centrifuged at 290rcf for 6 min at RT, washed twice with Washing Medium and finally plated in 50 μl/dome in Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (BME001-10, R&D systems), at the concentration of 5000 crypts/well. .. After the incubation at 37 °C for 10min to allow domes to solidify, 500 μl/well of pre-warmed tumor organoids medium IntestiCult Organoid Growth Medium (#06010, StemCell Technologies) were added.

    Article Title: Enterovirus D68 2A protease causes nuclear pore complex dysfunction and motor neuron toxicity
    Article Snippet: .. RD cells, HEK293T cells, and HeLa cells were from ATCC (CCL-136, CRL-3216, and CRM-CCL-2, respectively). iPSC lines were from the Cedars Sinai Biomanufacturing Center repository, and included lines CS0002, CS0003, CS88, CS0YX7, and CS8VTR. iPSCs were maintained in Essential 8 Flex media (Thermo Fisher) on 6-well tissue culture plates coated with Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (R&D Systems BME001), manually cleaned to remove differentiated cells, and passaged as colonies using ReLeSR passaging reagent (STEMCELL Technologies). ..

    Article Title: The Recycling Collagen Receptor uPARAP Is a Unique Mediator of Stromal Drug Delivery to Carcinoma Cells
    Article Snippet: These ADCs were produced by maleimide-based coupling to reduced IgG [mAb 2h9 or murine mAb anti–HyHEL-10 (cat. no. PA007154 .m1, Syd Labs)], and both had an average drug-to-antibody ratio (DAR) of 8, as determined by reverse-phase chromatography (Sterling Pharma Solutions). .. For cell culture and cell-based assays in vitro , reagents included collagenase type I (CAS no. 9001-12-1, cat. no. LS004197 ), DNAse I (CAS no. 9003-98-9, cat. no. LS002006 , Worthington Biochemical), neutral protease (dispase, CAS no. 9001-92-7, cat. no. LS02100, Worthington Biochemical), BSA (cat. no A3059, CAS no. 9048-46-8, Sigma-Aldrich), protease inhibitor cocktail III (cat. no. 535140, Sigma-Aldrich), skim milk powder (cat. no. 70166, Sigma-Aldrich), CellTiter 96 AQ ueous One Solution Cell Proliferation Assay reagent (MTS, cat. no. G358, Promega), and Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (cat. no. BME001, R&D Systems). .. The following cell lines were purchased from the indicated cell banks or commercial suppliers with designations as specified: human osteosarcoma SaOS-2 cell line (RRID:CVCL_0548, European Collection of Animal Cell Cultures; ECACC 89050205), human squamous cell lung carcinoma EBC-1 cell line (RRID:CVCL_2891, JCRB Cell Bank; JCRB0820), human osteosarcoma 143B cell line (RRID:CVCL_2270, ATCC; CRL-8303), human colorectal adenocarcinoma HT29 cell line (RRID:CVCL_A8EZ, ATCC; HTB-38), and luciferase-transfected HT29 (Luc-HT29) cells (RRID:CVCL_5J00, Revvity; IVISbrite HT29 Red F-luc, BW124353 ). uPARAP-deficient 143B cells were generated using CRISPR/Cas9-mediated gene editing as described ( , ).

    Article Title: Bridging the gap: organotypic models to study late-onset group B streptococcus infection.
    Article Snippet: .. The basement membrane matrix used was the Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (R&D Systems) and was diluted to a final concentration of 2.5 mg/mL. ..

    Article Title: Development, Expansion, and Histological Characterization of Patient-Derived Liver Organoids for Drug Screening and Disease Modeling
    Article Snippet: Cultrex organoid harvesting solution (R&D Systems, catalog number: BME001-10) 9. .. Cultrex UltiMatrix reduced growth factor basement membrane extract (R&D Systems, catalog number: BME001-10) 10. .. Diaminobenzidine tetrahydrochloride (DAB) (Biocare Medical, catalog number: DB801) 11.

    Article Title: Development, Expansion, and Histological Characterization of Patient-Derived Liver Organoids for Drug Screening and Disease Modeling
    Article Snippet: Cultrex organoid harvesting solution (R&D Systems, catalog number: BME001-10) 9. .. Cultrex UltiMatrix reduced growth factor basement membrane extract (R&D Systems, catalog number: BME001-10) 10. .. Diaminobenzidine tetrahydrochloride (DAB) (Biocare Medical, catalog number: DB801) 11.

    Article Title: The Recycling Collagen Receptor uPARAP Is a Unique Mediator of Stromal Drug Delivery to Carcinoma Cells
    Article Snippet: The genetic instability of cancer cells leads to cellular resistance against most targeted cancer drugs.. Cancer-associated fibroblasts (CAF) infiltrate all carcinomas and are genetically stable.. Using antibody–drug conjugates (ADC), we exploit the unique properties of a rapidly recycling endocytic receptor, uPARAP, to achieve highly efficient CAF-mediated drug delivery and killing of carcinomas.

    Concentration Assay:

    Article Title: Colorectal cancer patients-derived immunity-organoid platform unveils cancer-specific tissue markers associated with immunotherapy resistance.
    Article Snippet: Digestion was blocked with Washing Medium (DMEM F12 Advanced - 12634-010, Gibco, 15 mM Hepes - 15630-080, Gibco, 1% BSA - A1391, Applichem) then filtered through a 70 μm strainer. .. Tumoral crypts were centrifuged at 290rcf for 6 min at RT, washed twice with Washing Medium and finally plated in 50 μl/dome in Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (BME001-10, R&D systems), at the concentration of 5000 crypts/well. .. After the incubation at 37 °C for 10min to allow domes to solidify, 500 μl/well of pre-warmed tumor organoids medium IntestiCult Organoid Growth Medium (#06010, StemCell Technologies) were added.

    Article Title: Bridging the gap: organotypic models to study late-onset group B streptococcus infection.
    Article Snippet: .. The basement membrane matrix used was the Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (R&D Systems) and was diluted to a final concentration of 2.5 mg/mL. ..

    Passaging:

    Article Title: Enterovirus D68 2A protease causes nuclear pore complex dysfunction and motor neuron toxicity
    Article Snippet: .. RD cells, HEK293T cells, and HeLa cells were from ATCC (CCL-136, CRL-3216, and CRM-CCL-2, respectively). iPSC lines were from the Cedars Sinai Biomanufacturing Center repository, and included lines CS0002, CS0003, CS88, CS0YX7, and CS8VTR. iPSCs were maintained in Essential 8 Flex media (Thermo Fisher) on 6-well tissue culture plates coated with Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (R&D Systems BME001), manually cleaned to remove differentiated cells, and passaged as colonies using ReLeSR passaging reagent (STEMCELL Technologies). ..

    Cell Culture:

    Article Title: The Recycling Collagen Receptor uPARAP Is a Unique Mediator of Stromal Drug Delivery to Carcinoma Cells
    Article Snippet: These ADCs were produced by maleimide-based coupling to reduced IgG [mAb 2h9 or murine mAb anti–HyHEL-10 (cat. no. PA007154 .m1, Syd Labs)], and both had an average drug-to-antibody ratio (DAR) of 8, as determined by reverse-phase chromatography (Sterling Pharma Solutions). .. For cell culture and cell-based assays in vitro , reagents included collagenase type I (CAS no. 9001-12-1, cat. no. LS004197 ), DNAse I (CAS no. 9003-98-9, cat. no. LS002006 , Worthington Biochemical), neutral protease (dispase, CAS no. 9001-92-7, cat. no. LS02100, Worthington Biochemical), BSA (cat. no A3059, CAS no. 9048-46-8, Sigma-Aldrich), protease inhibitor cocktail III (cat. no. 535140, Sigma-Aldrich), skim milk powder (cat. no. 70166, Sigma-Aldrich), CellTiter 96 AQ ueous One Solution Cell Proliferation Assay reagent (MTS, cat. no. G358, Promega), and Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (cat. no. BME001, R&D Systems). .. The following cell lines were purchased from the indicated cell banks or commercial suppliers with designations as specified: human osteosarcoma SaOS-2 cell line (RRID:CVCL_0548, European Collection of Animal Cell Cultures; ECACC 89050205), human squamous cell lung carcinoma EBC-1 cell line (RRID:CVCL_2891, JCRB Cell Bank; JCRB0820), human osteosarcoma 143B cell line (RRID:CVCL_2270, ATCC; CRL-8303), human colorectal adenocarcinoma HT29 cell line (RRID:CVCL_A8EZ, ATCC; HTB-38), and luciferase-transfected HT29 (Luc-HT29) cells (RRID:CVCL_5J00, Revvity; IVISbrite HT29 Red F-luc, BW124353 ). uPARAP-deficient 143B cells were generated using CRISPR/Cas9-mediated gene editing as described ( , ).

    Article Title: The Recycling Collagen Receptor uPARAP Is a Unique Mediator of Stromal Drug Delivery to Carcinoma Cells
    Article Snippet: The genetic instability of cancer cells leads to cellular resistance against most targeted cancer drugs.. Cancer-associated fibroblasts (CAF) infiltrate all carcinomas and are genetically stable.. Using antibody–drug conjugates (ADC), we exploit the unique properties of a rapidly recycling endocytic receptor, uPARAP, to achieve highly efficient CAF-mediated drug delivery and killing of carcinomas.

    In Vitro:

    Article Title: The Recycling Collagen Receptor uPARAP Is a Unique Mediator of Stromal Drug Delivery to Carcinoma Cells
    Article Snippet: These ADCs were produced by maleimide-based coupling to reduced IgG [mAb 2h9 or murine mAb anti–HyHEL-10 (cat. no. PA007154 .m1, Syd Labs)], and both had an average drug-to-antibody ratio (DAR) of 8, as determined by reverse-phase chromatography (Sterling Pharma Solutions). .. For cell culture and cell-based assays in vitro , reagents included collagenase type I (CAS no. 9001-12-1, cat. no. LS004197 ), DNAse I (CAS no. 9003-98-9, cat. no. LS002006 , Worthington Biochemical), neutral protease (dispase, CAS no. 9001-92-7, cat. no. LS02100, Worthington Biochemical), BSA (cat. no A3059, CAS no. 9048-46-8, Sigma-Aldrich), protease inhibitor cocktail III (cat. no. 535140, Sigma-Aldrich), skim milk powder (cat. no. 70166, Sigma-Aldrich), CellTiter 96 AQ ueous One Solution Cell Proliferation Assay reagent (MTS, cat. no. G358, Promega), and Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (cat. no. BME001, R&D Systems). .. The following cell lines were purchased from the indicated cell banks or commercial suppliers with designations as specified: human osteosarcoma SaOS-2 cell line (RRID:CVCL_0548, European Collection of Animal Cell Cultures; ECACC 89050205), human squamous cell lung carcinoma EBC-1 cell line (RRID:CVCL_2891, JCRB Cell Bank; JCRB0820), human osteosarcoma 143B cell line (RRID:CVCL_2270, ATCC; CRL-8303), human colorectal adenocarcinoma HT29 cell line (RRID:CVCL_A8EZ, ATCC; HTB-38), and luciferase-transfected HT29 (Luc-HT29) cells (RRID:CVCL_5J00, Revvity; IVISbrite HT29 Red F-luc, BW124353 ). uPARAP-deficient 143B cells were generated using CRISPR/Cas9-mediated gene editing as described ( , ).

    Article Title: The Recycling Collagen Receptor uPARAP Is a Unique Mediator of Stromal Drug Delivery to Carcinoma Cells
    Article Snippet: The genetic instability of cancer cells leads to cellular resistance against most targeted cancer drugs.. Cancer-associated fibroblasts (CAF) infiltrate all carcinomas and are genetically stable.. Using antibody–drug conjugates (ADC), we exploit the unique properties of a rapidly recycling endocytic receptor, uPARAP, to achieve highly efficient CAF-mediated drug delivery and killing of carcinomas.

    Protease Inhibitor:

    Article Title: The Recycling Collagen Receptor uPARAP Is a Unique Mediator of Stromal Drug Delivery to Carcinoma Cells
    Article Snippet: These ADCs were produced by maleimide-based coupling to reduced IgG [mAb 2h9 or murine mAb anti–HyHEL-10 (cat. no. PA007154 .m1, Syd Labs)], and both had an average drug-to-antibody ratio (DAR) of 8, as determined by reverse-phase chromatography (Sterling Pharma Solutions). .. For cell culture and cell-based assays in vitro , reagents included collagenase type I (CAS no. 9001-12-1, cat. no. LS004197 ), DNAse I (CAS no. 9003-98-9, cat. no. LS002006 , Worthington Biochemical), neutral protease (dispase, CAS no. 9001-92-7, cat. no. LS02100, Worthington Biochemical), BSA (cat. no A3059, CAS no. 9048-46-8, Sigma-Aldrich), protease inhibitor cocktail III (cat. no. 535140, Sigma-Aldrich), skim milk powder (cat. no. 70166, Sigma-Aldrich), CellTiter 96 AQ ueous One Solution Cell Proliferation Assay reagent (MTS, cat. no. G358, Promega), and Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (cat. no. BME001, R&D Systems). .. The following cell lines were purchased from the indicated cell banks or commercial suppliers with designations as specified: human osteosarcoma SaOS-2 cell line (RRID:CVCL_0548, European Collection of Animal Cell Cultures; ECACC 89050205), human squamous cell lung carcinoma EBC-1 cell line (RRID:CVCL_2891, JCRB Cell Bank; JCRB0820), human osteosarcoma 143B cell line (RRID:CVCL_2270, ATCC; CRL-8303), human colorectal adenocarcinoma HT29 cell line (RRID:CVCL_A8EZ, ATCC; HTB-38), and luciferase-transfected HT29 (Luc-HT29) cells (RRID:CVCL_5J00, Revvity; IVISbrite HT29 Red F-luc, BW124353 ). uPARAP-deficient 143B cells were generated using CRISPR/Cas9-mediated gene editing as described ( , ).

    Article Title: The Recycling Collagen Receptor uPARAP Is a Unique Mediator of Stromal Drug Delivery to Carcinoma Cells
    Article Snippet: The genetic instability of cancer cells leads to cellular resistance against most targeted cancer drugs.. Cancer-associated fibroblasts (CAF) infiltrate all carcinomas and are genetically stable.. Using antibody–drug conjugates (ADC), we exploit the unique properties of a rapidly recycling endocytic receptor, uPARAP, to achieve highly efficient CAF-mediated drug delivery and killing of carcinomas.

    Proliferation Assay:

    Article Title: The Recycling Collagen Receptor uPARAP Is a Unique Mediator of Stromal Drug Delivery to Carcinoma Cells
    Article Snippet: These ADCs were produced by maleimide-based coupling to reduced IgG [mAb 2h9 or murine mAb anti–HyHEL-10 (cat. no. PA007154 .m1, Syd Labs)], and both had an average drug-to-antibody ratio (DAR) of 8, as determined by reverse-phase chromatography (Sterling Pharma Solutions). .. For cell culture and cell-based assays in vitro , reagents included collagenase type I (CAS no. 9001-12-1, cat. no. LS004197 ), DNAse I (CAS no. 9003-98-9, cat. no. LS002006 , Worthington Biochemical), neutral protease (dispase, CAS no. 9001-92-7, cat. no. LS02100, Worthington Biochemical), BSA (cat. no A3059, CAS no. 9048-46-8, Sigma-Aldrich), protease inhibitor cocktail III (cat. no. 535140, Sigma-Aldrich), skim milk powder (cat. no. 70166, Sigma-Aldrich), CellTiter 96 AQ ueous One Solution Cell Proliferation Assay reagent (MTS, cat. no. G358, Promega), and Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (cat. no. BME001, R&D Systems). .. The following cell lines were purchased from the indicated cell banks or commercial suppliers with designations as specified: human osteosarcoma SaOS-2 cell line (RRID:CVCL_0548, European Collection of Animal Cell Cultures; ECACC 89050205), human squamous cell lung carcinoma EBC-1 cell line (RRID:CVCL_2891, JCRB Cell Bank; JCRB0820), human osteosarcoma 143B cell line (RRID:CVCL_2270, ATCC; CRL-8303), human colorectal adenocarcinoma HT29 cell line (RRID:CVCL_A8EZ, ATCC; HTB-38), and luciferase-transfected HT29 (Luc-HT29) cells (RRID:CVCL_5J00, Revvity; IVISbrite HT29 Red F-luc, BW124353 ). uPARAP-deficient 143B cells were generated using CRISPR/Cas9-mediated gene editing as described ( , ).

    Article Title: The Recycling Collagen Receptor uPARAP Is a Unique Mediator of Stromal Drug Delivery to Carcinoma Cells
    Article Snippet: The genetic instability of cancer cells leads to cellular resistance against most targeted cancer drugs.. Cancer-associated fibroblasts (CAF) infiltrate all carcinomas and are genetically stable.. Using antibody–drug conjugates (ADC), we exploit the unique properties of a rapidly recycling endocytic receptor, uPARAP, to achieve highly efficient CAF-mediated drug delivery and killing of carcinomas.



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